Replication (1 and 2)
1. New DNA can only elongate from 5' to 3'. This means DNA polymerase III will move in the 3' to 5' direction with respect to the old DNA strand.
2. The leading and lagging strands are not the same on both replications forks in a replication bubble.
3. Sequence of Enzymes/Other Proteins
    Initiation - Helicase, Single Stranded Binding Proteins, Gyrase, Primase
    Elongation - DNA Polymerase III
    Termination - DNA Polymerase I, Ligase


Transcription (TFs (extra) and Spliceosomes)
4. In eukaryotes, RNA Polymerase II does not recognize the TATA box by itself. Transcription factors have to first bind to the TATA box and signal to RNA Poly. II.
5. Although the TATA box is read from 5' to 3', RNA Polymerase moves along the template (antisense) strand in the 3' to 5' direction. This causes an RNA molecule to elongate from 5' to 3'. This also means that the coding (sense) strand should be copied (by students, not RNA Poly II) from the 5' to 3' direction.
6. Transcription stops when RNA Poly II polymerizes an AAUAA sequence on the transcribed mRNA. This is equivalent to a TTATT sequence on the template strand or AATAA on the coding strand.
7. Sequence of Events
    Initiation - 5'TATA3' box, Transcription Factors, RNA Polymerase II
    Elongation - RNA Polymerase II (mRNA has U instead of T)
    Termination - AAUAA on mRNA
8. pre-RNA is modified by adding a 5' G cap and a 3' poly A tail to stabilize it. Introns are then cut out by spliceosomes (containing snRNP which contains snRNA and proteins), leaving the exons.

Translation (http://moodle.ayjscience.ca/mod/url/view.php?id=340&redirect=1)
9. mRNA is read 5' to 3' (which is also 5' to 3' on the coding strand). It is read codon by codon (3 by 3) starting from the codon AUG (ATG on coding strand), which codes for methyanine, until a stop codon is reached.
10. Prokaryotes have 30s and 50s ribosomal subunits; Eukaryotes have 40s and 60s subunits.
11. Translocation occurs when the ribosome shifts the tRNA from one site to the next. Because mRNA is bonded to tRNA at its anticodon, it also shifts along with it. This causes the mRNA molecule to move towards the 5' direction while the ribosome translocates towards the 3' direction.
12. There are 61 amino acid coding codons, but only 45 anti-codons. This is possible because of 3rd position wobble.
13. Sequence of Events
      Initiation - 5'AUG3', small and large ribosomal subunits, tRNA bound to methyanine
      Elongation - tRNA
      Termination - Stop codon

Extra: Control of Gene Expression: http://www.mcgrawhill.ca/school/applets/abbio/ch18/controlgene_control_of_.swf

The presentation on "Deaf by Design" made me realize that an entire culture exists within the Deaf community. Before, my limited knowledge of the deaf caused me to categorize them as part of an encompassing "handicapped" community, along with the blind and physically impaired. The article, however, changed that view. It also made me realize that other handicapped individuals are also part of a larger community with its own culture.

The presentation itself was quite interesting, mainly because of Mr Chung's delivery style. However, I would have employed more creative techniques to engage the class instead of the classic lecture/class discussion paradigm.