When transcribing from the student's perspective (not the RNA polymerase II), do the following:
1. Find 5'TATA3'. It will be on both coding and template strands.
2. Look 5' to 3' on each strand and find AATAA. This will be the coding strand.
3. Look for intronic sequence by focusing on one strand of the intronic sequence. Look for it by running in the same direction indicated by the intron along one strand of the DNA. Then run along the other strand.
*Remember to also transcribe AAUAA on the pre-mRNA, but not to include the TATA box
In the following relationships, = indicate the same, while # indicates complementary:
Anticodon (in order of translation, ie.5' to 3' on mRNA) # 5'mRNA3' = 5'coding strand3' # 3'template strand5'
Hence Anticodon (in order of 5'mRNA3') = 3'template strand5'
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